FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n02_1921.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n02_1921.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11726966
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG939280.800957383179929No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC876140.7471156648701803Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA231950.034.85479747
TCGCCGT233500.034.41358646
GGTCGCC237650.034.38756644
GTCGCCG238300.034.00046545
CCGTATC237100.033.5514349
GCCGTAT242850.033.34803448
GTGGTCG246900.033.16970442
CTCGGTG239600.032.89488638
ATCTCGG245500.032.30529836
GCGGCGC15200.032.2830351
TCTCGGT247950.031.8024537
CGTATCA252950.031.51838950
GATCTCG247850.031.40576435
GGTGGTC264500.031.12135141
TGGTCGC263300.030.94417843
TCGGTGG261750.030.9138839
CGGTGGT272100.030.2133940
AGATCTC261700.030.0512734
GTATCAT269350.029.06658451
CGTGTAG303750.028.78090516
Produced by FastQC (version 0.11.5)