FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n02_1920.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n02_1920.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10619122
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1810831.7052539748578084Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG898920.8465106625576013No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC111680.10516877007345805Illumina Single End PCR Primer 1 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA427800.038.14847647
CCGTATC419000.037.9389849
TCGCCGT428200.037.5243546
GCCGTAT442050.037.15623548
GGTCGCC457450.036.86886244
GTCGCCG458350.036.74405745
CGTATCA442750.036.5125350
AAAGGGG326150.036.35740766
GTGGTCG474350.035.68811442
AAGGGGG336850.035.51389767
TGGTCGC471450.035.22467843
CTCGGTG470500.035.20620738
GTATCAT444200.035.1799751
GGTGGTC489050.034.8084541
ATCTCGG474200.034.50457836
TCTCGGT478650.034.33003237
GATCTCG473050.034.30731235
AGGGGGG380350.033.90026568
TCGGTGG508450.033.7628639
CGGTGGT510900.033.73031240