Basic Statistics
Measure | Value |
---|---|
Filename | HVYT3AFXY_n02_1920.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 10619122 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 181083 | 1.7052539748578084 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 89892 | 0.8465106625576013 | No Hit |
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC | 11168 | 0.10516877007345805 | Illumina Single End PCR Primer 1 (100% over 49bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGCCGTA | 42780 | 0.0 | 38.148476 | 47 |
CCGTATC | 41900 | 0.0 | 37.93898 | 49 |
TCGCCGT | 42820 | 0.0 | 37.52435 | 46 |
GCCGTAT | 44205 | 0.0 | 37.156235 | 48 |
GGTCGCC | 45745 | 0.0 | 36.868862 | 44 |
GTCGCCG | 45835 | 0.0 | 36.744057 | 45 |
CGTATCA | 44275 | 0.0 | 36.51253 | 50 |
AAAGGGG | 32615 | 0.0 | 36.357407 | 66 |
GTGGTCG | 47435 | 0.0 | 35.688114 | 42 |
AAGGGGG | 33685 | 0.0 | 35.513897 | 67 |
TGGTCGC | 47145 | 0.0 | 35.224678 | 43 |
CTCGGTG | 47050 | 0.0 | 35.206207 | 38 |
GTATCAT | 44420 | 0.0 | 35.17997 | 51 |
GGTGGTC | 48905 | 0.0 | 34.80845 | 41 |
ATCTCGG | 47420 | 0.0 | 34.504578 | 36 |
TCTCGGT | 47865 | 0.0 | 34.330032 | 37 |
GATCTCG | 47305 | 0.0 | 34.307312 | 35 |
AGGGGGG | 38035 | 0.0 | 33.900265 | 68 |
TCGGTGG | 50845 | 0.0 | 33.76286 | 39 |
CGGTGGT | 51090 | 0.0 | 33.730312 | 40 |