FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n02_1918.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n02_1918.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12847953
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2595912.020485286644495Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1194600.9297979218946395No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC188360.1466070120275191Illumina Single End PCR Primer 1 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG48300.035.7757341
AAAGGGG446800.034.49851666
AAGGGGG460050.033.60368767
TGACTGG54100.032.4961132
GCGGCGC15300.032.3010331
GACTGGA59900.032.142393
AGGGGGG495050.031.72974868
GATAGAT142500.030.8966758
AGATAGA150250.029.5126427
CGCCGTA780600.029.35326447
CCGTATC771300.029.12173849
TCGCCGT784800.028.91968546
GCCGTAT799650.028.58833348
AAAAGGG544000.028.57249365
CGTATCA790400.028.5687850
GGTCGCC807600.028.5628144
GTCGCCG805950.028.47831745
ATAGATC157250.028.0876039
GTATCAT785000.027.96725851
GTGGTCG829650.027.79946742
Produced by FastQC (version 0.11.5)