FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n02_1845.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n02_1845.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11024996
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2325392.1091980441534854Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1514881.3740413148449215No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG50950.041.343551
AAGGGGG376900.038.75151467
AAAGGGG378100.038.69314666
CAGATCG199750.038.2520836
TGACTGG56050.037.427732
AGGGGGG411150.036.54508668
GACTGGA62800.034.168693
GATAGAT132850.033.825818
AGATAGA135200.033.7814757
CCAGATC70900.033.4220055
AGAGCAC70800.032.6252215
GAGCACA68550.031.95963516
GATCGAG32450.031.8164798
GCACACG34450.031.39149918
AAAAGGG491400.029.94277465
ACTGGAG75100.029.8756944
ATAGATC159600.028.2221729
GCAGATC77050.027.8469685
AGCACAC50800.027.21273817
CAGATAG58050.027.1933296
Produced by FastQC (version 0.11.5)