FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n02_1844.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n02_1844.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13708472
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC7497775.469442546186038Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1388971.0132201459068524No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC317260.2314335251952223Illumina Single End PCR Primer 1 (100% over 49bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC195000.14224780121373118Illumina Single End PCR Primer 1 (100% over 49bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC182440.13308558386375957Illumina Single End PCR Primer 1 (100% over 40bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC144380.10532173097045389Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG101000.051.8080141
TGACTGG108350.048.834082
AGATAGA293650.047.2797977
GACTGGA118700.046.444583
GATCGAG67850.046.0106548
GATAGAT301200.045.6763578
CAGATAG108850.044.1812676
GCACACG66150.044.07125518
AGAGCAC124500.043.6269315
GAGCACA119150.043.02982316
ACTGGAG136300.041.1901254
CGATCGG14500.039.3983571
AGCACAC86700.038.8726617
ACACGAG38350.038.23828520
AAAGGGG1083250.037.9074766
CACACGA40850.037.86852319
AAGGGGG1090000.037.6753867
CTGGAGT120200.036.6026275
AGGGGGG1145150.036.20619668
TAGATAG120650.036.2048766
Produced by FastQC (version 0.11.5)