FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n02_1843.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n02_1843.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14491087
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4146932.8617107881555053Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1641081.1324754312771705No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC194310.13408931986951703Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG65900.041.2185441
GATAGAT184700.038.4083638
AGATAGA190300.037.645937
GATCGAG48350.037.639678
TGACTGG73850.037.271072
AAAGGGG632100.036.70601366
AAGGGGG648600.035.70734867
GACTGGA82200.035.301783
AGGGGGG693500.034.0112468
AGAGCAC87600.032.9198315
GAGCACA84200.032.50286516
CGATCGG11450.032.4471631
ATAGATC221150.032.030419
AAAAGGG740100.031.55772265
CAGATAG80550.031.2420676
GCACACG43050.031.1360218
CCGTATC1134800.030.8318749
CGCCGTA1155100.030.82923947
ACTGGAG99550.030.5190774
TCGCCGT1161250.030.42776546
Produced by FastQC (version 0.11.5)