FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n02_163b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n02_163b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13413815
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3723162.775616034662771Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1711161.2756698970427132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG551300.040.4153966
AAGGGGG554350.040.319367
AGGGGGG603500.037.83030768
GATCGAG35250.036.9336978
AAAAGGG636200.035.22556365
AGATAGA150700.034.83517
GAGCACA65650.033.42428216
GCACACG32850.033.3459518
AGAGCAC70350.033.33060515
GATAGAT158500.032.767528
CCGTATC1010750.031.44429649
CGCCGTA1035350.031.32604247
GTGACTG48400.031.209131
CGTATCA1031400.031.01155350
TCGCCGT1040400.030.88805446
GCCGTAT1049100.030.84529748
GTATCAT1024600.030.41464251
GTCGCCG1079850.030.25931445
GGTCGCC1087850.030.22559544
CATTAAA1089800.029.60762255
Produced by FastQC (version 0.11.5)