FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n02_163a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n02_163a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14548825
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC6609444.542937316243751Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1938941.3327124355403273No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC156350.10746572317695759Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG80550.046.129941
GCACACG52350.043.98911318
GATCGAG54300.043.8919338
TGACTGG86100.043.279632
AGAGCAC108100.042.37877715
GAGCACA102950.042.25467716
GACTGGA90350.042.192983
AGATAGA203050.042.159067
AAGGGGG904650.038.1863767
AAAGGGG911000.037.91264366
AGCACAC72250.037.68567317
GATAGAT225050.037.617888
ACTGGAG104450.036.8620874
AGGGGGG961900.036.36856568
CAGATAG87550.035.66036
CACACGA34950.034.94795219
AAAAGGG1007800.034.42402665
TAACTGA96550.034.314992
ACACGAG35300.032.7176920
CCCCGTA33800.031.78868747
Produced by FastQC (version 0.11.5)