FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n02_1553.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n02_1553.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11264655
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2550912.2645256334969868Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1079190.9580320036432541No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG387000.035.4620467
GTGACTG44900.035.440271
AAAGGGG388500.035.43354466
AGGGGGG410700.033.8502968
GATAGAT130800.032.8570068
CGCCGTA666600.032.74622747
AGATAGA133800.032.5649647
CCGTATC657900.032.49818849
TCGCCGT666750.032.34517346
CGTATCA668700.032.12029350
GCCGTAT681050.031.92311148
GGTCGCC690300.031.83475344
GTCGCCG685150.031.829745
TGACTGG52150.031.6967162
GCACACG32150.031.67724818
GATCGAG31050.031.5597658
ATAGATC139200.031.0753889
TGGTCGC705100.031.04728143
GTGGTCG706150.031.02603542
GTATCAT680050.030.812551
Produced by FastQC (version 0.11.5)