FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n02_1551.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n02_1551.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11265831
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1085050.963133567332938Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG958790.8510601659123059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA270500.033.3297847
GGTCGCC277400.032.60124244
AAGGGGG182050.032.52932767
CCGTATC273600.032.3891549
TCGCCGT275700.032.38406846
GCACACG29750.032.35116618
GTCGCCG280250.032.0206545
AGAGCAC60850.031.74818215
AAAGGGG186000.031.72561566
GAGCACA57700.031.661916
GCCGTAT287100.031.4514348
CGTATCA284300.031.36724950
TGGTCGC291950.030.9643643
GTGGTCG295100.030.7407142
CTCGGTG287500.030.20195638
ATCTCGG297500.029.56426636
AGGGGGG208150.029.27438568
TCTCGGT299000.029.2052537
GGTGGTC311800.029.11655441
GATCTCG296300.028.93970535
Produced by FastQC (version 0.11.5)