FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16157398
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA2195901.3590678400074072TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG593460.3672992396424226No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG254240.15735206869323884TruSeq Adapter, Index 1 (100% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG192480.1191280922831758No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG88050.046.314461
TGACTGG92450.044.0222662
AAAAAGG461050.037.20872570
ACTGGAG115200.035.6903084
GGAGTTC110400.034.9769257
TCACAGA560750.032.33387431
GTCACAG579900.031.34029430
CACAGAT587700.030.63550632
GACTGGA135850.030.3946023
TGGAGTT140400.029.607716
GAGTTCA129900.029.2691978
ACAGATC636750.028.15545333
CTGGAGT157100.026.4609345
GCTTGAA598300.026.18169460
CTTGAAA622200.025.77708861
AGTTCAG148300.025.710139
AAAAAAG750100.024.90538469
TTGAAAA680750.024.25684562
AGCTCGG34550.024.02437441
ATGCCGT676150.023.81485448
Produced by FastQC (version 0.11.5)