FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n01_201.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n01_201.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11746752
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG867180.7382295974240368TruSeq Adapter, Index 3 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG203850.032.03721258
GCCGTCT209650.031.06769450
TGCCGTC241200.030.06560749
TGCTTGA218000.029.92562559
TATGCCG256500.029.71933647
CCGTCTT228450.029.6752351
ATGCCGT245200.029.6465148
GCTTGAA235350.029.48902960
AAAAAGG323400.029.05736470
GTATGCC273500.028.84464346
CGTATGC272250.028.37285645
CTCGTAT249050.028.16281543
CATCTCG268450.028.05692340
TCTGCTT246750.027.28988557
CGTCTTC242300.027.15564352
TCGTATG258400.027.1031344
TCTCGTA262300.026.99416442
ATCTCGT272050.026.94039341
GCATCTC285650.026.69833839
CTTCTGC255450.026.31913255
Produced by FastQC (version 0.11.5)