FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n01_1922.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n01_1922.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10326263
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG1016600.9844800582747117TruSeq Adapter, Index 13 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG231450.032.61692470
CTACACG54550.029.64087312
CGACGCT54800.028.9947317
CCTACAC55900.028.61199811
CCCTACA56500.028.24634710
ACGACGC56450.028.14723216
ACACGAC57900.027.92589814
TCCCTAC59500.027.0574429
GCTTGAA290450.027.01531860
CTGCTTG286350.026.71781758
TGCCGTC304800.026.4443749
GCCGTCT293500.026.43698350
TGCTTGA293000.026.24281559
TATGCCG313700.025.88365747
ATGCCGT313250.025.78676648
CCGTCTT302650.025.70722251
GTATGCC321200.025.42092746
GACGCTC62150.025.3968118
CACGACG66850.024.92007315
CTCGTAT317900.024.68272243
Produced by FastQC (version 0.11.5)