FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n01_1921.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n01_1921.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11726966
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG778580.6639227912829286TruSeq Adapter, Index 12 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT203050.031.3881750
TGCCGTC218950.031.26676649
TATGCCG227550.031.16187147
ATGCCGT224550.030.54935648
GTATGCC236550.030.47931146
CTGCTTG209400.030.0013858
CCGTCTT218400.029.87117851
CTCGTAT211450.029.54550743
AAAAAGG247050.029.08373370
TGCTTGA222650.028.1845559
CGTATGC256150.027.95569645
TCTCGTA227350.027.72551542
GCTTGAA233550.027.61842560
ATCTCGT230300.027.55273641
AATCTCG234250.027.26684640
AGATCGG328550.026.9481641
GCACACG330400.026.82033512
GATCGGA328750.026.7656882
ACACGTC333450.026.606514
TCGTATG261450.026.57252344
Produced by FastQC (version 0.11.5)