FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n01_1920.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n01_1920.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10619122
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG2282962.1498575870961836TruSeq Adapter, Index 11 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG169770.15987197434966846No Hit
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT137000.12901254924842187TruSeq Adapter, Index 11 (100% over 48bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG360500.040.59076770
GCTTGAA484300.035.17923760
CTGCTTG491100.034.0152758
TGCTTGA496050.033.81694859
GCCGTCT508300.033.42885650
TGCCGTC523250.033.41016849
CTTGAAA515900.033.37057561
AAAAAAG460250.033.1849669
CTACACG73950.033.17537712
CCGTCTT515600.033.15241251
TATGCCG534100.032.9689147
ATGCCGT531200.032.91673748
CCTACAC75250.032.64875411
ACGACGC74700.032.6081916
GTATGCC546300.032.3607846
CGACGCT75700.032.08497217
ACACGAC77550.032.0414814
CCCTACA76800.031.8532710
CGTCTTC542900.031.46598852
CTCGTAT553100.031.41841943
Produced by FastQC (version 0.11.5)