FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n01_1918.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n01_1918.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12847953
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG1173290.91321162211599TruSeq Adapter, Index 10 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATG348040.2708914019221583TruSeq Adapter, Index 10 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG218300.16991033513276396No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATG147850.11507669743187883TruSeq Adapter, Index 10 (97% over 45bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG452750.044.28623670
CTACACG101400.036.6890912
CCTACAC104300.035.56830611
CCCTACA104950.035.4480610
ACACGAC106000.035.4270914
CGACGCT105100.035.0977717
ACGACGC106750.034.719216
CTGCTTG295950.034.29478558
AAAAAAG626650.034.02383469
TCCCTAC110000.033.6935359
GCTTGAA331350.032.4371760
TGCTTGA307450.032.3860459
CACGACG116200.032.3474315
GACGCTC114650.031.68582218
TACACGA119400.031.21669613
ATGCCGT322300.031.17630648
TGCCGTC334200.031.10296249
GCCGTCT274050.030.82898550
CGTCTGC102450.030.71115155
CCGTCTT311400.029.80632251
Produced by FastQC (version 0.11.5)