FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n01_1845.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n01_1845.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11024996
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG2646842.4007627757869483TruSeq Adapter, Index 6 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130990.11881183448955446No Hit
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT114800.10412702190549547TruSeq Adapter, Index 6 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG428750.041.402570
AAAAAAG546450.033.42614469
TAGATCG222250.030.3228786
GTAGATC66500.026.9544035
AAGATCG252950.026.5734526
ACACGAC84950.026.49031814
CCTACAC83700.026.42598511
TCCCTAC84950.026.3668449
CTACACG84150.026.3262612
GAGATCG192450.026.2318426
CGACGCT83850.026.0866617
CCCTACA85200.026.08397510
ACGACGC84350.026.01500916
GCCGGCT23400.024.82822256
TGCCGGC28500.024.4376655
ACACTCT92100.023.9943391
GCGTGAA54050.023.9591466
TACACGA94350.023.70273813
CTTGAAA856050.023.6643567
TGAAAAA937600.023.66206269
Produced by FastQC (version 0.11.5)