FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n01_1844.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n01_1844.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13708472
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG3446222.5139344487117166TruSeq Adapter, Index 5 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG2129151.5531636202780297TruSeq Adapter, Index 5 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATG554920.40480076845909596TruSeq Adapter, Index 5 (97% over 41bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG404960.2954085619462184No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATG363380.2650769538720289TruSeq Adapter, Index 5 (97% over 49bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG220690.1609880371787607TruSeq Adapter, Index 5 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGGATG184870.13485821030965378TruSeq Adapter, Index 5 (97% over 41bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG182370.13303452055050338No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGGATG154900.11299581747695878TruSeq Adapter, Index 5 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG1031300.055.879270
AAAAAAG1263700.047.23678269
ACACTCT162400.038.9690931
TGAAAAA1467100.038.4991763
CTTGAAA1159750.038.3356961
TTGAAAA1199900.038.19342862
CCTACAC174700.037.20130511
CCCTACA175050.037.1669110
CTACACG176200.037.0037812
GCTTGAA1184450.036.8317760
TCCCTAC177050.036.8262639
ACGACGC178200.036.66703416
GAAAAAA1570850.036.64933864
ACACGAC180700.036.64380314
CGACGCT177450.036.5065817
TGCTTGA1150850.035.8939159
CTGCTTG1175100.035.209958
CACGACG188850.034.78455415
TACACGA189650.034.47173713
GACGCTC184800.034.31602518
Produced by FastQC (version 0.11.5)