FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n01_1843.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n01_1843.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14491087
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG4543973.1356998960809497TruSeq Adapter, Index 4 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG230470.1590425894206556No Hit
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT214620.14810483161132082TruSeq Adapter, Index 4 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG676100.045.81217670
AAAAAAG841250.037.95834769
TGAAAAA1123300.032.7742963
TTGAAAA1097550.032.62802562
CTACACG106700.032.40694412
CTTGAAA1099950.032.3627461
CCTACAC108250.032.136911
CCCTACA109600.031.58139410
ACACGAC111050.031.5471114
GCTTGAA1126200.031.37490360
CGACGCT110350.031.14484817
GAAAAAA1190950.031.13006464
TCCCTAC111650.031.0328779
ACGACGC111000.030.96246716
TGCTTGA1151100.030.27055759
CTGCTTG1170850.029.69130158
GCCGTCT1249050.028.17424450
TGCCGTC1280250.028.05351649
CCGTCTT1258300.028.0422351
TCTGCTT1248850.028.00200857
Produced by FastQC (version 0.11.5)