FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n01_163b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n01_163b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13413815
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG2600561.938717657877345TruSeq Adapter, Index 2 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATG465110.34673953681335246TruSeq Adapter, Index 2 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG186910.1393414177845751No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATG136670.1018874943481776TruSeq Adapter, Index 2 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG613350.045.14123570
AAAAAAG788750.036.52276669
CTGCTTG551400.035.8364158
TGCTTGA560950.035.22630759
GCTTGAA597150.035.1363160
CTTGAAA651300.032.84435761
TCTGCTT617800.032.78367657
GCCGTCT591050.032.01726550
TGCCGTC687850.031.79076249
ATGCCGT685850.031.4651448
CGTCTGC121200.031.36011155
CCGTCTT628500.031.26797551
CGTCTTC618250.030.7560752
CTTCTGC649600.030.75306355
TGAAAAA910500.030.53255563
TTCTGCT583800.030.44851756
TATGCCG768800.030.06016247
GTATGCC807000.029.70847746
CGACGCT78650.029.54699317
TTGAAAA754200.029.52346462
Produced by FastQC (version 0.11.5)