FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n01_163a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n01_163a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14548825
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG7386425.076987316845175TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA407590.2801532082487761TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG281290.19334207401628653No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC178170.12246349791134337TruSeq Adapter, Index 1 (100% over 50bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT176780.1215080942962748TruSeq Adapter, Index 1 (100% over 48bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149250.10258560399207496No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG971500.048.55756870
AAAAAAG1174900.041.053869
CGACGCT143100.034.33756617
TTGAAAA1692800.034.17841762
CCCTACA146750.034.15186310
CTACACG146700.034.13964512
TGAAAAA1719800.034.13815763
CTTGAAA1692800.034.09571561
ACGACGC145950.034.0267416
CCTACAC147400.033.90628411
TCCCTAC148100.033.816929
GAAAAAA1766350.033.41681764
ACACGAC153800.033.06414414
ACACTCT146900.032.5203821
GCTTGAA1782300.032.18445660
TCGGGGG11550.032.1340874
GACGCTC154150.031.24042518
TACACGA162050.030.99219913
CACGACG163350.030.89542415
TGCTTGA1860250.030.59689359
Produced by FastQC (version 0.11.5)