FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n01_1553.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n01_1553.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11264655
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG2889692.5652716394776407TruSeq Adapter, Index 9 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG121780.10810806012256921No Hit
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT119650.10621718996276407TruSeq Adapter, Index 9 (100% over 48bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG451700.041.13510570
CTTGAAA695000.032.57289561
AAAAAAG587800.032.50346469
GCTTGAA701800.032.061560
TGCTTGA722350.030.87806159
TGAAAAA748850.030.73507563
CTGCTTG726350.030.66465258
TTGAAAA754100.030.16398662
GCCGTCT750300.029.87720350
TGCCGTC762550.029.67262549
CCGTCTT763550.029.41374251
GAAAAAA789150.029.30730264
ATGCCGT775650.029.1896648
TATGCCG776350.029.15909247
GTATGCC788600.028.75939446
TCTGCTT776950.028.74865557
CGACGCT71800.028.61263317
CTTCTGC786950.028.49871855
CGTCTTC792650.028.44869652
CTACACG73050.028.36244812
Produced by FastQC (version 0.11.5)