FastQCFastQC Report
Fri 18 Oct 2019
HVYT3AFXY_n01_1551.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYT3AFXY_n01_1551.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11265831
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG609680.5411762345804761TruSeq Adapter, Index 7 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATG123730.109827672721169TruSeq Adapter, Index 7 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT130800.032.05548550
TGCCGTC157100.031.54571549
CTGCTTG148950.031.15718758
TATGCCG168600.030.55717347
ATGCCGT159300.030.01153648
GTATGCC178450.029.96882446
CCGTCTT151550.029.00594351
AAAAAGG271100.028.59548670
TGCTTGA162550.028.31352859
CATCTCG196400.028.22727440
GCTTGAA174250.028.03934360
CGTATGC183500.028.01861845
CTCGTAT166050.027.78021643
TCTCGTA176550.026.36627442
TCGTATG170550.026.26741244
ATCTCGT196150.025.9977741
TCTGCTT189950.025.40845557
CGTCTTC170150.025.38262252
TGCCGGC50150.024.98419649
AGATCGG423350.024.8240321
Produced by FastQC (version 0.11.5)