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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2019-08-15, 01:08 based on data in: /beegfs/mk5636/logs/html/HVYH3BGXB/merged

        Welcome! Not sure where to start?   Watch a tutorial video   (6:06)

        General Statistics

        Showing 22/22 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HVYH3BGXB_n01_10G-HB7
        65.2%
        49%
        1.6
        HVYH3BGXB_n01_10R-MYB44
        42.5%
        47%
        1.4
        HVYH3BGXB_n01_11G-ABF1
        56.4%
        48%
        1.5
        HVYH3BGXB_n01_11R-GBF3
        49.2%
        48%
        1.7
        HVYH3BGXB_n01_12G-ZAT6
        42.5%
        47%
        1.6
        HVYH3BGXB_n01_1G-ABF3
        56.5%
        49%
        1.3
        HVYH3BGXB_n01_1R-EV
        60.5%
        49%
        2.9
        HVYH3BGXB_n01_2G-HB7
        62.4%
        50%
        1.7
        HVYH3BGXB_n01_2R-MYB44
        39.9%
        47%
        1.8
        HVYH3BGXB_n01_3G-ABF1
        62.0%
        49%
        1.7
        HVYH3BGXB_n01_3R-GBF3
        53.3%
        46%
        2.0
        HVYH3BGXB_n01_4G-ZAT6
        49.4%
        48%
        2.6
        HVYH3BGXB_n01_5G-ABF3
        63.2%
        50%
        1.7
        HVYH3BGXB_n01_5R-EV
        69.3%
        50%
        1.7
        HVYH3BGXB_n01_6G-HB7
        49.4%
        47%
        1.6
        HVYH3BGXB_n01_6R-MYB44
        80.2%
        49%
        1.0
        HVYH3BGXB_n01_7G-ABF1
        65.5%
        50%
        1.7
        HVYH3BGXB_n01_7R-GBF3
        71.8%
        51%
        1.5
        HVYH3BGXB_n01_8G-ZAT6
        44.9%
        47%
        1.7
        HVYH3BGXB_n01_9G-ABF3
        60.8%
        49%
        1.6
        HVYH3BGXB_n01_9R-EV
        42.3%
        47%
        1.3
        HVYH3BGXB_n01_undetermined
        96.2%
        45%
        27.2

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 22/22 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        27,201,185
        43.2
        1R-EV
        2,908,437
        4.6
        2R-MYB44
        1,842,487
        2.9
        3R-GBF3
        2,042,391
        3.2
        5R-EV
        1,656,049
        2.6
        6R-MYB44
        1,049,099
        1.7
        7R-GBF3
        1,473,149
        2.3
        9R-EV
        1,330,524
        2.1
        10R-MYB44
        1,448,553
        2.3
        11R-GBF3
        1,676,382
        2.7
        1G-ABF3
        1,314,902
        2.1
        2G-HB7
        1,675,171
        2.7
        3G-ABF1
        1,684,583
        2.7
        4G-ZAT6
        2,646,749
        4.2
        5G-ABF3
        1,736,254
        2.8
        6G-HB7
        1,647,116
        2.6
        7G-ABF1
        1,682,630
        2.7
        8G-ZAT6
        1,669,575
        2.7
        9G-ABF3
        1,582,076
        2.5
        10G-HB7
        1,571,603
        2.5
        11G-ABF1
        1,511,635
        2.4
        12G-ZAT6
        1,577,675
        2.5

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        GGGGGG
        26427364.0
        97.2
        CAAAAA
        20509.0
        0.1
        AAAAAA
        13916.0
        0.1
        NGGGGG
        13209.0
        0.1
        TGGGGG
        11606.0
        0.0
        CAAAAC
        11540.0
        0.0
        CGGGGG
        9165.0
        0.0
        CAAAAT
        8246.0
        0.0
        AAAAAT
        8151.0
        0.0
        AAAAAC
        6637.0
        0.0
        CAACAA
        6626.0
        0.0
        NNGGGG
        6305.0
        0.0
        CAAACA
        5965.0
        0.0
        GGGGGC
        5710.0
        0.0
        AAAAAG
        5619.0
        0.0
        TATAAA
        5372.0
        0.0
        TAAAAA
        5103.0
        0.0
        CAATAA
        4888.0
        0.0
        GACGCC
        4616.0
        0.0
        AATAAT
        4472.0
        0.0

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        4.0
        63,907,183
        62,928,225
        43.2
        40.7

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms
        22
        0
        0

        The mean quality value across each base position in the read. See the FastQC help.

        Created with Highcharts 5.0.6Position (bp)Phred ScoreChart context menuExport PlotMean Quality Scores102030405060700510152025303540Created with MultiQC

        Per Sequence Quality Scores
        22
        0
        0

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

        Created with Highcharts 5.0.6Mean Sequence Quality (Phred Score)CountChart context menuExport PlotPer Sequence Quality Scores0510152025303505M10M15M20M25M30MCreated with MultiQC

        Per Base Sequence Content
        1
        0
        21

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content
        0
        2
        20

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

        Created with Highcharts 5.0.6%GCCountChart context menuExport PlotPer Sequence GC Content01020304050607080901000246810Created with MultiQC

        Per Base N Content
        22
        0
        0

        The percentage of base calls at each position for which an N was called. See the FastQC help.

        Created with Highcharts 5.0.6Position in Read (bp)Percentage N-CountChart context menuExport PlotPer Base N Content0102030405060700123456Created with MultiQC

        Sequence Length Distribution
        22
        0
        0

        All samples have sequences of a single length (76bp).

        Sequence Duplication Levels
        0
        8
        14

        The relative level of duplication found for every sequence. See the FastQC help.

        Created with Highcharts 5.0.6Sequence Duplication Level% of LibraryChart context menuExport PlotSequence Duplication Levels123456789>10>50>100>500>1k>5k>10k+020406080100Created with MultiQC

        Overrepresented sequences
        0
        19
        3

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        Created with Highcharts 5.0.6Percentage of Total SequencesChart context menuExport PlotOverrepresented sequencesTop over-represented sequenceSum of remaining over-represented sequencesHVYH3BGXB_n01_10G-HB7HVYH3BGXB_n01_10R-MYB44HVYH3BGXB_n01_11G-ABF1HVYH3BGXB_n01_11R-GBF3HVYH3BGXB_n01_12G-ZAT6HVYH3BGXB_n01_1G-ABF3HVYH3BGXB_n01_1R-EVHVYH3BGXB_n01_2G-HB7HVYH3BGXB_n01_2R-MYB44HVYH3BGXB_n01_3G-ABF1HVYH3BGXB_n01_3R-GBF3HVYH3BGXB_n01_4G-ZAT6HVYH3BGXB_n01_5G-ABF3HVYH3BGXB_n01_5R-EVHVYH3BGXB_n01_6G-HB7HVYH3BGXB_n01_6R-MYB44HVYH3BGXB_n01_7G-ABF1HVYH3BGXB_n01_7R-GBF3HVYH3BGXB_n01_8G-ZAT6HVYH3BGXB_n01_9G-ABF3HVYH3BGXB_n01_9R-EVHVYH3BGXB_n01_undetermined0%2%4%6%8%10%12%14%16%18%20%22%Created with MultiQC

        Adapter Content
        22
        0
        0

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        Created with Highcharts 5.0.6Position% of SequencesChart context menuExport PlotAdapter Content510152025303540455055600123456Created with MultiQC

        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.