FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27201185
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3692451.3574592430440071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT10100.044.0060963
TACTCTA10400.043.4090654
ACGTCTG3000.041.99273715
CACGTCT3150.039.99469814
TCTAGTT11400.038.987217
GCACACG3550.037.4590711
TTGTACT12200.035.606171
TGTACTC13150.034.3333972
ACTCTAG14550.033.4330375
CACACGT4150.032.8864212
AGTGGGG3000.032.67447366
TGTTACC13850.031.83687613
CTCTAGT14300.031.8153116
GGGGGAT2100.031.66963260
GTTACCT14600.030.44117214
TACCTCT14500.030.40814216
CTAGTTG14500.030.1698598
GAGTGGG3300.029.70258965
CTAATGC15100.029.66794221
GGGAGTG3500.028.0049463
Produced by FastQC (version 0.11.5)