FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_9R-EV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_9R-EV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1330524
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA60600.45545965348990325No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT26650.20029702583343104No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG26350.19804227507358002No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA25950.19503594072711203No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT24060.1808310109400507No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT19480.146408482672992No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA19370.1455817407277133No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG19240.14460468206511118No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA17350.13039975227804984No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA17240.12957301033277116No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG17000.12776920972489034No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC16550.12438708358511383No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT15530.11672093100162041No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGC13980.10507138540905689TruSeq Adapter, Index 12 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT16950.045.8400421
TACTCTA17850.043.7248574
GTACTCT17750.043.576833
TCTAGTT18200.042.8856057
TGTACTC18250.042.3765752
ACTCTAG21750.041.8400465
CTCTAGT18850.041.5924726
CTAGTTG20650.036.6123928
TATGCCG3000.036.16070646
GTATGCC3050.035.56790545
CGTATGC3150.035.54969444
ACGTCTG3100.034.9942315
GCACACG3050.034.4231411
TAGTTGT22350.033.9841659
TCGTATG3200.032.8070943
ACCTCTA24100.031.5093317
TACCTCT24000.031.34899916
TGTTACC24200.031.23569513
GTTACCT24150.031.15545514
TTACCTC24950.030.15534815
Produced by FastQC (version 0.11.5)