Basic Statistics
Measure | Value |
---|---|
Filename | HVYH3BGXB_n01_9G-ABF3.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1582076 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA | 5789 | 0.3659116249788253 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGC | 5010 | 0.31667252394954476 | TruSeq Adapter, Index 3 (97% over 36bp) |
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG | 2804 | 0.17723548046996476 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT | 2584 | 0.16332970097517438 | No Hit |
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA | 2531 | 0.15997967227870216 | No Hit |
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT | 2088 | 0.13197848902328332 | No Hit |
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT | 2007 | 0.12685863384565596 | No Hit |
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA | 1790 | 0.11314247861670362 | No Hit |
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA | 1667 | 0.10536788371734354 | No Hit |
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA | 1601 | 0.10119614986890642 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 655 | 0.0 | 60.37369 | 46 |
GTATGCC | 660 | 0.0 | 59.916313 | 45 |
CGTATGC | 665 | 0.0 | 58.93957 | 44 |
GCACACG | 675 | 0.0 | 58.07006 | 11 |
CTCGTAT | 695 | 0.0 | 56.40255 | 42 |
TCGTATG | 705 | 0.0 | 55.595486 | 43 |
ACGTCTG | 725 | 0.0 | 54.54623 | 15 |
CACGTCT | 720 | 0.0 | 54.438965 | 14 |
AGTCACT | 735 | 0.0 | 53.339767 | 28 |
GTCACTC | 740 | 0.0 | 52.979366 | 29 |
AGCACAC | 765 | 0.0 | 51.23667 | 10 |
CAGTCAC | 770 | 0.0 | 50.91523 | 27 |
GCCGTCT | 780 | 0.0 | 50.698425 | 49 |
CCAGTCA | 800 | 0.0 | 49.441902 | 26 |
CACACGT | 795 | 0.0 | 49.30477 | 12 |
TCCAGTC | 820 | 0.0 | 48.662865 | 25 |
CATCTCG | 810 | 0.0 | 48.39784 | 39 |
GAGCACA | 845 | 0.0 | 46.390263 | 9 |
TCTAGTT | 1315 | 0.0 | 46.045357 | 7 |
TCACTCA | 855 | 0.0 | 45.85493 | 30 |