FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_9G-ABF3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_9G-ABF3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582076
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA57890.3659116249788253No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGC50100.31667252394954476TruSeq Adapter, Index 3 (97% over 36bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG28040.17723548046996476No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT25840.16332970097517438No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA25310.15997967227870216No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT20880.13197848902328332No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT20070.12685863384565596No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA17900.11314247861670362No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA16670.10536788371734354No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA16010.10119614986890642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6550.060.3736946
GTATGCC6600.059.91631345
CGTATGC6650.058.9395744
GCACACG6750.058.0700611
CTCGTAT6950.056.4025542
TCGTATG7050.055.59548643
ACGTCTG7250.054.5462315
CACGTCT7200.054.43896514
AGTCACT7350.053.33976728
GTCACTC7400.052.97936629
AGCACAC7650.051.2366710
CAGTCAC7700.050.9152327
GCCGTCT7800.050.69842549
CCAGTCA8000.049.44190226
CACACGT7950.049.3047712
TCCAGTC8200.048.66286525
CATCTCG8100.048.3978439
GAGCACA8450.046.3902639
TCTAGTT13150.046.0453577
TCACTCA8550.045.8549330