FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_8G-ZAT6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_8G-ZAT6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669575
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGC42860.256712037494572TruSeq Adapter, Index 9 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA29000.1736968989113996No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT20500.12278573888564455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7100.054.21917746
GTATGCC7300.052.7337245
CGTATGC7500.051.79410644
GCACACG7550.051.45417811
CACACGT7750.050.5779212
ACGTCTG7850.049.93361315
CTCGTAT7800.049.3607542
AGCACAC8100.048.82452810
CACGTCT8100.048.3953514
TCGTATG8500.045.70068443
CACTATA8550.045.0281631
TATCTCG8900.044.04784839
TCTCGTA9150.042.4618141
GCCGTCT9100.042.3028849
TCCAGTC9200.042.22598325
CCAGTCA9200.042.22598326
CTATAAT9250.042.00276633
ACACGTC9500.041.2621713
GAGCACA9600.041.2031029
AGTCACT9450.041.1113628
Produced by FastQC (version 0.11.5)