FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_7R-GBF3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_7R-GBF3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473149
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA72240.49037809481593514No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG32060.21762903820319604No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT31120.2112481493725346No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC26920.18273779502277096TruSeq Adapter, Index 7 (97% over 36bp)
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA26750.18158380448956624No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT23560.15992951154296No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA23390.1587755210097553No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT22660.15382014989658208No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT19950.13542418316137744No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG19620.13318408389103886No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA19000.12897541253464517No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA18880.1281608309817948No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA18840.12788930379751132No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC18480.12544555913896013No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT18110.1229339326843381No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTT17920.12164417855899166No Hit
GTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCT17050.11573846230082632No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA16990.11533117152440113No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA16430.11152979094443263No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA16030.10881451910159802No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA15260.10358762080414133No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC14740.1000577674084563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3650.058.4881343
GTATGCC3800.056.1793945
TATGCCG3900.054.7407546
CTCGTAT3900.053.8470242
ACGTCTG3900.053.84153415
CGTATGC3950.053.15999244
GCACACG3950.053.1581911
GCCGTCT4100.051.21685449
AGCACAC4100.051.2133810
GTACTCT15700.050.1600383
GCGATCT4200.050.000837
CACGTCT4200.049.99740614
TACTCTA15850.049.6853374
CACACGT4250.049.40584612
ATGCCGT4300.048.83467547
ACTCCAG4500.048.2197223
ACCAGGC4400.047.72965632
CAGTCAC4400.047.72965627
CTGCTTG4400.047.72317557
AGTCACC4650.045.9162728
Produced by FastQC (version 0.11.5)