FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_7G-ABF1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_7G-ABF1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682630
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA41280.24533022708498006No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAGCATCTCGTATGC35500.21097924083131764TruSeq Adapter, Index 7 (97% over 35bp)
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA22110.13140143703606855No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA19120.11363163618858572No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA18810.11178928225456577No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG18770.11155155916630513No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT17750.10548962041565882No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT17640.10483588192294205No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT17550.10430100497435563No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTT17520.10412271265816013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4950.061.50465846
CGTATGC5150.059.7956244
GTATGCC5200.058.547745
GCACACG5300.058.1084711
CACGTCT5650.055.12988714
CACACGT5700.054.0306812
ACGTCTG5850.053.24352315
TCGTATG5800.053.09438743
ACTACAG5750.052.95535332
CTACAGC5800.052.49884433
CTCGTAT5800.052.4957242
AGCACAC5950.052.34867510
CATCTCG5950.051.77125539
GCCGTCT6050.050.90040249
CCAGTCA6150.050.65380526
CACTACA6100.049.9124831
TCCAGTC6150.048.94637325
CAGTCAC6450.047.7579827
TACAGCA6400.047.5756634
CTCCAGT6500.046.84929724