Basic Statistics
Measure | Value |
---|---|
Filename | HVYH3BGXB_n01_7G-ABF1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1682630 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA | 4128 | 0.24533022708498006 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAGCATCTCGTATGC | 3550 | 0.21097924083131764 | TruSeq Adapter, Index 7 (97% over 35bp) |
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA | 2211 | 0.13140143703606855 | No Hit |
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA | 1912 | 0.11363163618858572 | No Hit |
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA | 1881 | 0.11178928225456577 | No Hit |
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG | 1877 | 0.11155155916630513 | No Hit |
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT | 1775 | 0.10548962041565882 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT | 1764 | 0.10483588192294205 | No Hit |
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT | 1755 | 0.10430100497435563 | No Hit |
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTT | 1752 | 0.10412271265816013 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 495 | 0.0 | 61.504658 | 46 |
CGTATGC | 515 | 0.0 | 59.79562 | 44 |
GTATGCC | 520 | 0.0 | 58.5477 | 45 |
GCACACG | 530 | 0.0 | 58.10847 | 11 |
CACGTCT | 565 | 0.0 | 55.129887 | 14 |
CACACGT | 570 | 0.0 | 54.03068 | 12 |
ACGTCTG | 585 | 0.0 | 53.243523 | 15 |
TCGTATG | 580 | 0.0 | 53.094387 | 43 |
ACTACAG | 575 | 0.0 | 52.955353 | 32 |
CTACAGC | 580 | 0.0 | 52.498844 | 33 |
CTCGTAT | 580 | 0.0 | 52.49572 | 42 |
AGCACAC | 595 | 0.0 | 52.348675 | 10 |
CATCTCG | 595 | 0.0 | 51.771255 | 39 |
GCCGTCT | 605 | 0.0 | 50.900402 | 49 |
CCAGTCA | 615 | 0.0 | 50.653805 | 26 |
CACTACA | 610 | 0.0 | 49.91248 | 31 |
TCCAGTC | 615 | 0.0 | 48.946373 | 25 |
CAGTCAC | 645 | 0.0 | 47.75798 | 27 |
TACAGCA | 640 | 0.0 | 47.57566 | 34 |
CTCCAGT | 650 | 0.0 | 46.849297 | 24 |