FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_6G-HB7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_6G-HB7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647116
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA84380.5122893590979627No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC63290.3842473754125393TruSeq Adapter, Index 3 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT38980.23665607036784295No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA36680.2226922693969338No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG36220.21989950920275197No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT29130.17685457490547113No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT28730.17442608778009563No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA27950.1696905378856134No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA24900.15117332355462518No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA22850.13872732703707571No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG22380.13587385466475949No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC21030.12767771061661717No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT16710.10145004966256171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC8200.059.75003445
CGTATGC8250.058.96371544
ACGTCTG8400.057.91606515
TATGCCG8400.057.9107946
GCACACG9050.053.75145311
CACACGT9650.050.7720512
CACGTCT9600.050.6765614
CTCGTAT9600.050.31197742
TCGTATG9800.049.99492643
GAGCACA10050.049.450729
AGCACAC10100.048.5099310
GCCGTCT10250.048.48288749
ACACGTC10250.047.46292513
CAGTCAC10350.047.00719527
TCCAGTC10450.046.8908925
CCAGTCA10450.046.8908926
AGTCACT10550.046.44783828
TCTCGTA10850.045.1621941
TACTCTA20550.044.621054
GTACTCT20600.044.172963
Produced by FastQC (version 0.11.5)