Basic Statistics
Measure | Value |
---|---|
Filename | HVYH3BGXB_n01_5G-ABF3.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1736254 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTCGTATGC | 9445 | 0.5439872276752135 | TruSeq Adapter, Index 6 (97% over 36bp) |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA | 5222 | 0.30076244604764046 | No Hit |
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA | 2404 | 0.13845900427011257 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT | 2286 | 0.13166276362790238 | No Hit |
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG | 2211 | 0.1273431191519213 | No Hit |
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA | 1904 | 0.1096613744302389 | No Hit |
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA | 1828 | 0.1052841346945781 | No Hit |
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT | 1824 | 0.1050537536558591 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 1120 | 0.0 | 63.429436 | 46 |
CGTATGC | 1145 | 0.0 | 62.04451 | 44 |
GTATGCC | 1150 | 0.0 | 61.774757 | 45 |
AGTCACG | 1170 | 0.0 | 60.724022 | 28 |
CTCGTAT | 1175 | 0.0 | 60.470848 | 42 |
GCACACG | 1180 | 0.0 | 60.211147 | 11 |
TCGTATG | 1190 | 0.0 | 59.698296 | 43 |
ACGTCTG | 1200 | 0.0 | 59.20592 | 15 |
GCCGTCT | 1205 | 0.0 | 58.955162 | 49 |
CCAGTCA | 1225 | 0.0 | 58.281662 | 26 |
CAGTCAC | 1240 | 0.0 | 57.57664 | 27 |
CACGTCT | 1240 | 0.0 | 57.297703 | 14 |
TCCAGTC | 1250 | 0.0 | 57.116028 | 25 |
CATCTCG | 1255 | 0.0 | 56.61776 | 39 |
ACGACAT | 1260 | 0.0 | 56.39309 | 35 |
AGCACAC | 1260 | 0.0 | 56.38659 | 10 |
CACACGT | 1280 | 0.0 | 55.50715 | 12 |
GACGACA | 1305 | 0.0 | 54.4485 | 34 |
GACATCT | 1320 | 0.0 | 54.094936 | 37 |
GAGCACA | 1345 | 0.0 | 53.087925 | 9 |