FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_5G-ABF3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_5G-ABF3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1736254
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTCGTATGC94450.5439872276752135TruSeq Adapter, Index 6 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA52220.30076244604764046No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA24040.13845900427011257No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT22860.13166276362790238No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG22110.1273431191519213No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA19040.1096613744302389No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA18280.1052841346945781No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT18240.1050537536558591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11200.063.42943646
CGTATGC11450.062.0445144
GTATGCC11500.061.77475745
AGTCACG11700.060.72402228
CTCGTAT11750.060.47084842
GCACACG11800.060.21114711
TCGTATG11900.059.69829643
ACGTCTG12000.059.2059215
GCCGTCT12050.058.95516249
CCAGTCA12250.058.28166226
CAGTCAC12400.057.5766427
CACGTCT12400.057.29770314
TCCAGTC12500.057.11602825
CATCTCG12550.056.6177639
ACGACAT12600.056.3930935
AGCACAC12600.056.3865910
CACACGT12800.055.5071512
GACGACA13050.054.448534
GACATCT13200.054.09493637
GAGCACA13450.053.0879259