FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_4G-ZAT6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_4G-ZAT6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2646749
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTAT105810.3997734579289536TruSeq Adapter, Index 19 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA45260.17100223708406048No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT30820.11644474032105046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC14300.057.75502446
TATGCCG14300.057.75502448
GTATGCC14500.056.95839747
AGTCACG15050.054.88930528
GCACACG15250.054.84560411
GTCACGT15650.052.78392829
ACGTCTG15750.052.6610815
CTCGTAT15650.052.10211644
TCGTATG16250.051.4704945
TCACGTG16050.051.4684430
CACACGT16350.051.15568512
CACGTGA16250.050.8292231
CAGTCAC16650.050.4555827
GCCGTCT16450.050.4192351
AGCACAC16650.050.2339610
CACGTCT16600.050.17540414
ACGTGAT17250.047.8853132
CCAGTCA18050.046.34557726
ACACGTC18100.045.82386813
GAGCACA18350.045.771719
Produced by FastQC (version 0.11.5)