FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_3R-GBF3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_3R-GBF3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042391
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA207631.0166025996001744No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT111210.5445088624068556No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA99590.48761476132630827No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT76440.3742672191563711No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCTCGTATGC75960.37191703253686487TruSeq Adapter, Index 7 (97% over 35bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG74580.3651602460057844No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA72680.3558574239702388No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA65390.32016396468648756No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT63830.3125258581730922No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA60390.2956828540666307No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC42940.21024377800333038No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG42500.20808944026878298No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGAT39890.19531030052521775No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTA35280.17273871653370976No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT30100.14737628593153806No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG30060.14718043704657924No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG28790.1409622349491356No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT27040.1323938462321857No Hit
ATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCTCCTA26280.12867271741796746No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC25820.12642045524094064No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCT25290.12382545751523583No Hit
CTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGAC24640.12064291313465443No Hit
GTTTGGTACTATCTGCTATGTATCCTGTCCTACTATCTAACCTCTGTAGG23740.1162363132230802No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG23360.11437574881597108No Hit
CCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTAC23180.11349442883365625No Hit
GTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAG22450.10992018668315713No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGAC22370.10952848891323944No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAG21840.10693349118753462No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAG21580.10566047343530205No Hit
GTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATA21520.10536670010786377No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG21390.10473019123174748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC9900.057.2670845
CGTATGC10100.056.47957644
TATGCCG10150.055.8565646
CTCGTAT10600.053.82071742
ACGTCTG11250.051.64061715
GCACACG11200.051.2449511
CACACGT11250.051.01718512
TCGTATG11250.050.39502743
ATGCCGT11400.050.03892547
TTGTACT54350.049.334431
TACTCTA54650.049.248534
CACGTCT11950.048.32277714
TGTACTC55350.048.308352
CAGTCAC11900.048.23644327
GTACTCT55750.047.9629173
GCCGTCT12100.047.1441149
AGTCACC12050.047.0550628
AGCACAC12300.046.66206710
ACACGTC12250.046.56797413
ACTCTAG60350.046.2788665
Produced by FastQC (version 0.11.5)