FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_3G-ABF1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_3G-ABF1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684583
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA60390.3584863435045943No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA35720.21204060589475257No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGC33050.19619098613722208TruSeq Adapter, Index 2 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT28830.17114027625827877No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG28200.16740047833796257No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT26330.15629980832051613No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA22800.13534506759239526No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA22460.13332676395285953No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA18730.11118478578971769No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA18700.11100670017446454No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA18300.10863222530442251No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT18160.10780115909990781No Hit
GTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCT17190.10204305754005591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3950.061.13278644
GTATGCC4000.060.36862645
ACGTCTG4400.054.88382715
TATGCCG4400.054.8805746
GCACACG4400.054.0884111
CACGTCT4650.051.93308314
TCGTATG4850.049.7885643
CTCGTAT4950.048.7899742
AGCACAC5050.047.12513710
CACCTAT5200.047.1118131
CACACGT5150.046.89103712
CATCTCG5400.044.72546839
AGTCACC5400.044.7228128
GTCACCT5500.043.90966829
CTATACA5600.043.12556534
GCCGTCT5600.043.12044549
TACTCTA17950.042.7012444
GTACTCT17900.042.6249923
CAGTCAC5700.042.37023527
TCACCTA5800.041.63847730
Produced by FastQC (version 0.11.5)