FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_2R-MYB44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_2R-MYB44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842487
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG37430.20314933022593917No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA36070.19576800270503944No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT22100.1199465722146208No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC20820.11299944043024455No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG20420.11082846174762699No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC19750.10719207245424255No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT18470.10024494066986633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG751.4551915E-1146.666361
TACTCTA12350.032.875174
ACTCTAG17900.031.8712965
GTACTCT13050.031.1117523
TGTACTC13450.030.4425892
TATGCCG3000.030.32901846
CTCTAGT14500.029.4480846
TCTAGTT14700.029.047437
CGTATGC3450.028.40175644
GTATGCC3350.028.20494345
TTGTACT14550.027.4224991
CTAGTTG15300.027.2220448
ACGTCTG4000.025.3713915
GCGGGGC851.3094972E-424.705721
CACGTCT4050.024.19408814
GGGCCCC1451.7309503E-824.1377729
TAGTTGT17800.023.9886059
CTAATGC16850.023.67936721
ACCTCTA17650.023.19846217
GCTGACT6500.023.1536941
Produced by FastQC (version 0.11.5)