FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_2G-HB7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_2G-HB7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675171
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA59470.3550085334571814No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA29130.17389269513381023No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC27660.16511747158946757TruSeq Adapter, Index 3 (97% over 37bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG26280.15687950662947245No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT23100.13789636998252716No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA22540.13455342767992046No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT22320.13324012891818207No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA21380.12762876148166366No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT21130.1261363765251428No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA20110.12004744590253771No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA20030.11956988271645103No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT17700.10566085492167666No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG17670.10548176872689415No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTT17090.10201943562776576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG252.3893749E-456.00938430
GTATGCC3300.054.08382845
CGTATGC3200.053.58673544
TATGCCG3300.053.0233646
CACCTAG3300.051.96909731
GCACACG3400.050.4360811
ACCTAGC3450.049.71105632
CTCGTAT3750.045.7300842
TCGTATG3850.044.53962343
ACGTCTG3850.044.53962315
CACACGT3900.043.96991312
GTACTCT15250.042.2252433
TACTCTA15550.041.8607184
TCACCTA4100.041.8362830
TAGCTAT4200.041.66991835
CACGTCT4250.040.3488614
TCTAGTT16600.038.791267
CCTAGCT4600.038.04417433
GCTATCT4650.037.6373537
GTCACCT4600.037.28885729
Produced by FastQC (version 0.11.5)