FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_1R-EV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_1R-EV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908437
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA58760.20203291321077266No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC52450.18033741146877172TruSeq Adapter, Index 7 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT32190.11067800333993826No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG31700.10899324963889538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7350.051.4239346
CGTATGC7350.051.4230544
GTATGCC7700.049.5401445
ATGCCGT8550.044.20654347
GCACACG8650.043.6947311
TCGTATG8650.043.6947343
CTCGTAT8700.043.04283542
ACGTCTG9250.040.86188515
CACACGT9550.039.94335612
CACGTCT9650.039.16812514
CAGTCAC9750.039.13140527
TACTCTA17200.038.66134
AGTCACC10500.037.00302528
AGCACAC10400.036.67875710
GTACTCT18650.035.843123
TCCAGTC10650.035.4958625
GCCGTCT10750.035.1596249
CCAGTCA11100.034.05625526
GAGCACA11550.033.93759
TCTAGTT19850.033.85267
Produced by FastQC (version 0.11.5)