FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_1G-ABF3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_1G-ABF3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314902
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA52760.40124663282891043No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT27060.20579480447972548No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA25970.19750521331627757No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG22470.1708872600391512No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATGC22360.17005069579329868TruSeq Adapter, Index 7 (97% over 37bp)
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT20110.15293915440086028No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA17880.13597971559857694No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT17050.1296674581071441No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA17030.1295153555169891No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA14680.11164330117377569No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG13500.10266924835463022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2700.057.03186444
GTATGCC3050.049.3397945
GCACACG3300.046.66420711
CTCGTAT3250.046.30701442
TATGCCG3250.046.3034946
TCGTATG3250.046.3034943
ACGTCTG3500.043.9976815
CACACGT3600.043.74769612
TACTCTA15100.042.6500054
TCTAGTT15200.041.910477
AGCACAC3700.041.6194310
GTACTCT15400.041.591883
GAGCACA4050.038.8897979
CACGTCT4000.038.4979714
CGGAATA4000.037.62587734
AGTCACC4250.036.23752228
GCCGTCT4250.036.23200649
ACTCTAG20650.035.933045
CAGTCAC4400.035.0008227
ACCTACT6250.034.72213433