Basic Statistics
Measure | Value |
---|---|
Filename | HVYH3BGXB_n01_1G-ABF3.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1314902 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA | 5276 | 0.40124663282891043 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT | 2706 | 0.20579480447972548 | No Hit |
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA | 2597 | 0.19750521331627757 | No Hit |
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG | 2247 | 0.1708872600391512 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATGC | 2236 | 0.17005069579329868 | TruSeq Adapter, Index 7 (97% over 37bp) |
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT | 2011 | 0.15293915440086028 | No Hit |
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA | 1788 | 0.13597971559857694 | No Hit |
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT | 1705 | 0.1296674581071441 | No Hit |
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA | 1703 | 0.1295153555169891 | No Hit |
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA | 1468 | 0.11164330117377569 | No Hit |
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG | 1350 | 0.10266924835463022 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 270 | 0.0 | 57.031864 | 44 |
GTATGCC | 305 | 0.0 | 49.33979 | 45 |
GCACACG | 330 | 0.0 | 46.664207 | 11 |
CTCGTAT | 325 | 0.0 | 46.307014 | 42 |
TATGCCG | 325 | 0.0 | 46.30349 | 46 |
TCGTATG | 325 | 0.0 | 46.30349 | 43 |
ACGTCTG | 350 | 0.0 | 43.99768 | 15 |
CACACGT | 360 | 0.0 | 43.747696 | 12 |
TACTCTA | 1510 | 0.0 | 42.650005 | 4 |
TCTAGTT | 1520 | 0.0 | 41.91047 | 7 |
AGCACAC | 370 | 0.0 | 41.61943 | 10 |
GTACTCT | 1540 | 0.0 | 41.59188 | 3 |
GAGCACA | 405 | 0.0 | 38.889797 | 9 |
CACGTCT | 400 | 0.0 | 38.49797 | 14 |
CGGAATA | 400 | 0.0 | 37.625877 | 34 |
AGTCACC | 425 | 0.0 | 36.237522 | 28 |
GCCGTCT | 425 | 0.0 | 36.232006 | 49 |
ACTCTAG | 2065 | 0.0 | 35.93304 | 5 |
CAGTCAC | 440 | 0.0 | 35.00082 | 27 |
ACCTACT | 625 | 0.0 | 34.722134 | 33 |