FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_12G-ZAT6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_12G-ZAT6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577675
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA62890.3986245582898886No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGC49650.31470359864991204TruSeq Adapter, Index 10 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT30310.19211814854136625No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG27960.17722281204937645No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA23410.14838290522446004No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT20820.13196634287796916No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT18060.11447224555120668No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA17840.11307778851791402No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT17700.11219040676945505No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG16740.10610550335145072No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC15860.10052767521828007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC6550.054.49796745
CGTATGC6550.053.96367344
GCACACG7100.050.77080511
ACTCGGC7100.049.78812432
TATGCCG7100.049.7833946
TCGTATG7450.047.44457243
ACGTCTG7600.047.43212515
ACACGTC7550.047.28269213
CACACGT7600.046.97013512
TCACTCG7650.046.6675830
GTCACTC7750.046.5155629
CTCGTAT7750.045.16216742
AGCACAC8200.043.9600910
AGTCACT8150.043.80314628
CACGTCT8300.043.43182814
TACTCTA17300.042.4823344
GCCGTCT8450.042.24398849
CAGTCAC8600.041.9180927
CGGCAAT8650.041.27509735
ACTCTAG21900.041.229765