FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_11R-GBF3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_11R-GBF3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676382
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA38630.23043673816588342No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC23470.14000388932832733TruSeq Adapter, Index 2 (97% over 36bp)
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA21550.1285506525362358No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT18420.1098794904741282No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG18130.10814957450032271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG3400.051.46738411
TATGCCG3900.044.8676646
CACACGT3950.044.29972512
CGTATGC4100.043.5325844
GTATGCC4050.043.205945
AGCACAC4300.040.6951410
ACGTCTG4500.039.6618315
CACGTCT4500.038.8853114
ATGCCGT4550.038.45799647
CTCGTAT5000.035.6999142
TACTCTA11900.035.584964
GCCGTCT5350.034.66970449
TCGTATG5100.034.30954443
GTACTCT12350.034.288343
TCCAGTC5200.033.65376325
TCTAGTT13500.031.6276637
GAGCACA5800.030.7748669
CAGTCAC5700.030.70442627
CACCCAA5750.029.8251231
CCAGTCA6150.029.02431526
Produced by FastQC (version 0.11.5)