FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_11G-ABF1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_11G-ABF1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511635
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA64670.4278149156377036No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC35400.23418351652349942TruSeq Adapter, Index 10 (97% over 36bp)
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA33190.21956358512471597No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT30680.20295904765369946No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG26190.17325611010594488No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT25740.17027920099759533No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT21430.14176702709318056No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA20490.13554859473351702No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA20470.13541628766203484No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA19330.12787478458754925No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC15780.1043902793994582No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG15760.104257972327976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC5050.058.9065245
GCACACG5300.056.1297711
TATGCCG5350.055.6033546
CGTATGC5450.054.58310744
AGCACAC5450.053.94273810
TCACTCG5450.053.3040930
ACGTCTG5600.053.12105615
AGTCACT5900.051.01822728
CTCGTAT5850.050.8542942
GTCACTC6050.049.17478629
GCCGTCT6000.048.99636549
CACGTCT6100.048.19473614
TCGTATG6300.047.2187243
CACACGT6300.046.6647512
TCCAGTC6250.046.47962625
GAGCACA6750.044.0722669
GTACTCT19200.043.7467543
TACTCTA19450.043.7242624
CCAGTCA6750.043.55520626
CAGTCAC6850.042.9207827
Produced by FastQC (version 0.11.5)