FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_10R-MYB44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_10R-MYB44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448553
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA77240.5332217737286796No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT37870.26143330620281063No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA36380.2511471792885728No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG34640.23913519215382523No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT30010.20717226086998541No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT28840.199095235038No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG26720.18445994036807767No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA26020.17962753175065047No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA25910.1788681532536262No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGC24290.16768457902472328TruSeq Adapter, Index 9 (97% over 36bp)
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA23700.16361154890432036No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC20920.14441998325225242No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT20840.1438677079816893No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG20070.13855205850251942No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC17380.11998180252983494No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG15570.10748657453334466No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTA15110.1043109917276068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4600.050.9734444
GTATGCC4700.050.63350345
TATGCCG4900.048.56682646
TCGTATG5000.046.8955643
TTGTACT19100.046.18481
CTCGTAT5150.045.53281442
TACTCTA19650.045.239014
GCACACG5300.044.90295811
TGTACTC19850.044.4290432
ACTCTAG23400.044.4204065
ACGTCTG5350.043.8306515
GTACTCT20250.043.5529373
CTCTAGT20450.043.4692656
TGCCGTC5600.043.12091448
ATGCCGT5550.042.87882247
CACACGT5600.042.49744412
TCTAGTT21200.041.2696767
CACGTCT5750.040.78437814
GCCGTCT5850.040.08167649
TATCTCG5850.039.48753439