FastQCFastQC Report
Thu 15 Aug 2019
HVYH3BGXB_n01_10G-HB7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVYH3BGXB_n01_10G-HB7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571603
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA91950.585071420708665No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAATCTCGTATGC50780.32310958938103324TruSeq Adapter, Index 6 (97% over 36bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG44120.2807324750589048No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT37610.23930980024853607No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA31790.20227754719226165No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA29590.18827910101978682No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT28330.18026180912100576No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT27530.17517146505828762No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG26740.17014475029635348No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC23600.1501651498501848No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA22420.14265689235767556No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA20530.130630954509504No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA18610.11841412875898047No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA16290.10365213097709791No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA16290.10365213097709791No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT16260.10346124307474597No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTA16170.10288857936769018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5900.059.90945446
CGTATGC6000.058.91096544
GTATGCC6000.058.91096545
CTCGTAT6100.057.95258342
CACGTCT6300.056.10746414
GCACACG6350.055.66567211
ACGTCTG6350.055.66567215
TCGTATG6400.055.22902743
CACTCCC6700.053.2801231
AGTCACT6900.051.23181528
GTCACTC6900.051.23181529
TCCAGTC6850.051.09482225
CACACGT7050.050.13858812
CAGTCAC7100.049.78866227
CCAGTCA7150.048.95098526
GCCGTCT7300.048.89937249
GAGCACA7350.048.0967039
GTACTCT19550.047.9794733
TCACTCC7450.047.9209230
AGCACAC7400.047.76716210
Produced by FastQC (version 0.11.5)