FastQCFastQC Report
Wed 1 Nov 2017
HVY3KBCXY_l02n02_input_ctrl_replicate_2_root_acetyl_abs.352000000a594e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVY3KBCXY_l02n02_input_ctrl_replicate_2_root_acetyl_abs.352000000a594e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13259638
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT32750.013.80915910
CTTCCGA33250.013.3984979
GTGTGCT42050.011.1296082
GTGCTCT42050.010.9155774
TGTGCTC43000.010.5174423
CGTGTGC45150.010.2657811
TCCGATC44400.010.08445911
TCTTCCG53400.08.5955068
TGCTCTT54900.08.401645
GCTCTTC59750.07.94560676
TCGTGTA47800.07.625522645
CCGATCT63500.07.40551212
CTCTTCC73100.06.58686737
AGAGCGT120400.06.26038245
CGTGTAG47000.05.79255345
GTCGTGT63850.05.74393144
GTGTAGG37400.05.65508145
AAGAGCG142800.05.37289944
GAGCGTC122250.05.208588645
GAAGAGC176350.05.12900545
Produced by FastQC (version 0.11.2)