FastQCFastQC Report
Wed 1 Nov 2017
HVY3KBCXY_l02n02_input_ctrl_replicate_1_root_acetyl_abs.352000000a5924.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVY3KBCXY_l02n02_input_ctrl_replicate_1_root_acetyl_abs.352000000a5924.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13331720
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT35100.015.12820410
CTTCCGA37600.014.06259
GTGTGCT42250.012.6213022
CGTGTGC44250.012.3050841
TGTGCTC44450.012.249723
TCCGATC47050.011.71625911
GTGCTCT46450.011.6254044
TCTTCCG55750.09.645748
TGCTCTT57550.09.4613385
CCGATCT60600.09.17079312
GCTCTTC60600.09.1336646
TCGTGTA113400.09.0476245
CGTGTAG94850.07.87559345
TGTAGGG60950.07.826086545
GTCGTGT143250.07.319371745
GTAGGGA50800.07.263779645
CTCTTCC82050.07.15722137
GTGTAGG77350.06.690368745
TAGGGAA46350.06.40776745
AGCGTCG245700.06.117215645
Produced by FastQC (version 0.11.2)