FastQCFastQC Report
Wed 1 Nov 2017
HVY3KBCXY_l02n01_input_ctrl_replicate_2_root_acetyl_abs.351000000a5941.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVY3KBCXY_l02n01_input_ctrl_replicate_2_root_acetyl_abs.351000000a5941.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13259638
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT49800.07.138467345
ACGTCTG37550.06.950648345
CACACGT68000.06.74991845
CGTCTGA31700.06.529888645
ACACGTC59950.05.81727744
AGAGCAC127650.05.78136345
AGCACAC101250.05.466600445
GAGCACA117550.05.359356445
TCGGAAG220500.05.152998445
GAAGAGC176100.05.046787345
GCACACG94850.05.00521145
Produced by FastQC (version 0.11.2)