FastQCFastQC Report
Wed 1 Nov 2017
HVY3KBCXY_l02n01_input_ctrl_replicate_1_root_acetyl_abs.351000000a5927.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVY3KBCXY_l02n01_input_ctrl_replicate_1_root_acetyl_abs.351000000a5927.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13331720
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT114150.08.96837845
ACGTCTG87800.08.48226645
CGTCTGA70500.07.850990345
ACACGTC136650.07.55755845
CACACGT159850.06.728119445
GTCTGAA68000.06.551409245
AGCACAC227950.06.39607945
GCACACG200650.06.0776945
GAGCACA267800.05.822387745
TCTGAAC64650.05.77720845
AAGAGCA353300.05.483248745
AGAGCAC301450.05.35158345
CTGAACT61350.05.24444945
GAAGAGC401800.05.17416745
GAACTCC55900.05.071508445