FastQCFastQC Report
Wed 1 Nov 2017
HVY3KBCXY_l02n01_chip_ctrl_replicate_3_root_h3k27ac.351000000a590d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVY3KBCXY_l02n01_chip_ctrl_replicate_3_root_h3k27ac.351000000a590d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9458960
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC57400.08.46678645
ACGTCTG35150.08.25735945
CACGTCT50200.07.97798245
CACACGT71650.06.751480644
GAGCACA125750.06.54862145
GCACACG90950.06.481497345
CGTCTGA29200.06.47251745
AGCACAC108400.05.853243445
AGAGCAC138400.05.85252444
AAGAGCA170800.05.49319445
ATCGGAA278650.05.361493645
GATCGGA322800.05.339148545
GAAGAGC184100.05.18189744
GTCTGAA33500.05.17157345