FastQCFastQC Report
Wed 1 Nov 2017
HVY3KBCXY_l01n02_input_ctrl_replicate_2_shoot_acetyl_abs.352000000a5917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVY3KBCXY_l01n02_input_ctrl_replicate_2_shoot_acetyl_abs.352000000a5917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4735341
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA15250.025.3769959
TTCCGAT16450.023.25149510
CCGATCT17150.022.95840812
TCCGATC17400.022.62854611
CGTGTGC17600.021.9874291
TCTTCCG19450.020.4751038
GTGCTCT18950.020.302964
TGTGCTC20600.019.0044123
GTGTGCT21650.017.7703882
GCTCTTC23150.016.6194846
TGCTCTT28200.013.8028725
CTCTTCC32950.012.0862147
CGATCTA10100.011.13823713
CGATCTG10750.08.3718113
CGATCTT13000.08.1343413
CGATCTC13450.07.52762713
AGAGCGT7452.5790447E-45.13416345
Produced by FastQC (version 0.11.2)